X-Ray Computed Tomography for Root Quantification

• 作者: Bente Foereid
• 来源期刊: 土壤科学期刊(英文)
• 学科: 医学
• 关键词: ROOTS 3D IMAGE X-RAY COMPUTED TOMOGRAPHY
• 年，卷（期）: 2015,（7）
• 页码范围: 145-148
• 页数: 4页
• 分类号: R73

摘要

Soil cores from a field growing barley and barley mutants without root hairs under conventional and minimum tillage were sampled. They were X-ray scanned to produce a 3D image and then the roots were washed out and weight and length were determined by conventional means. Root volume and surface area were then calculated from the 3D images using state of the art software and methodology, and the measured and calculated measures were correlated. The only strong and significant correlation was between measured weight and calculated volume for mutants without root hairs. It is concluded that the software cannot segment out very small roots, but segmentation accuracy also depends on root structure in some unknown way. Any study using X-ray computed tomography to quantify roots as they grow in situ should start with a calibration for the conditions in question.