Direct Solid-State Fermentation of Soybean Processing Residues for the Production of Fungal Chitosan by Mucor rouxii

• 作者: Andro Mondala Brian Young James Atkinson Jan Pekarovic Ramea Al-Mubarak Shaun Shields Yurguen Dos Santos Senger
• 来源期刊: 材料科学与化学工程(英文)
• 学科: 医学
• 关键词: SOLID-STATE FERMENTATION FUNGAL CHITOSAN Biopolymers Bioconversion Agro-Industrial RESIDUES
• 年，卷（期）: 2015,（2）
• 页码范围: 11-21
• 页数: 11页
• 分类号: R73

摘要

The feasibility of utilizing soybean-processing residues such as soybean meal and hulls as substrates for chitosan production by the fungus Mucor rouxii ATCC 24905 via solid-state fermentation (SSF) was investigated. The effects of the type of soybean-based substrate, length of cultivation period, substrate moisture content, substrate pH, incubation temperature and extraction conditions on chitosan yield were determined. The results showed that a maximum fungal chitosan yield of up to 3.44% by dry substrate weight (34.4 g/kg) could be achieved using a pure soybean meal substrate with an initial moisture content of 50% (w/w) and pH of 5 - 6 incubated for six days at 25&degC. A more severe heat treatment (autoclaving vs. refluxing) resulted in higher chitosan extraction yields regardless of the strength of extraction reagents. Fourier transform infrared (FTIR) analysis of the fungal chitosan revealed its degree of deacetylation (DDA) to be between 55% and 60%.