Optimization of the Solid-phase Fermentation Medi- um of Metarhizium flavoviride by Response Surface Method

• 作者: Chen Dexin Li Maoye Li Shiguang Lin Huafeng Pan Jing Xu Amei
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: 发酵培养基 响应面法 优化 固相 绿僵菌 生产成本 技术支持 培养基配方
• 年，卷（期）: 2015,（1）
• 页码范围: 35-40
• 页数: 6页
• 分类号: S476.12

摘要

Rice flour,puparium of yellow mealworm,peanut shell and sunflower seed shell are made into the culture medium,and the optimal solid-phase fermentation formula for M. flavoviride is figured out. In the solid-phase fermentation,Design-Expert is applied to analyze the significance of all components on the sporulation,and response surface method used to optimize the fermentation medium of M. flavoviride. The results show that puparium of yellow mealworm is the most significant component for the sporulation; and the optimal medium formula for the fermentation of M. flavoviride: 0. 25-g rice flour,0. 14-g yellow mealworm puparium,1. 00-g peanut shell,and 0. 25-g sunflower seedshell. In this optimal medium,the sporulation achieves 100. 013 × 108 spores / m L fungal liquid,31. 7% higher than that of the initial design. Application of the optimal formula will reduce the production cost,obtain higher yield of spores,produce no pollution,and offer technical support for the future large-scale production.