Membrane Separator Interface for Mass-Spectrometric Analysis of Desflurane, Propofol and Fentanyl in Plasma and Cerebrospinal Fluid

• 作者: A. V. Polegaev A. Yu. Elizarov V. A. Elokhin V. I. Nikolaev V. N. Sokolov V. Yu. Cherebillo
• 来源期刊: 医学物理学、临床工程、放射肿瘤学(英文)
• 学科: 医学
• 关键词: MEMBRANE MASS-SPECTROMETER ANESTHESIA DESFLURANE FENTANYL
• 年，卷（期）: 2015,（4）
• 页码范围: 263-267
• 页数: 5页
• 分类号: R73

摘要

Mass-spectrometric interface for the measurement of anaesthetic agent concentration in biological fluids (blood plasma and cerebrospinal fluid) is described. Sampling of biological fluids was performed during balanced inhalational (desflurane, fentanyl) anaesthesia and total intravenous (propofol, fentanyl) anaesthesia. The described method for drug concentration measurement in biologic fluids does not require long-term sample processing before injecting the sample into mass-spectrometer interface, in contrast to chromatographic methods. A hydrophobic membrane was used in the interface to separate anaesthetic agents from biological fluids: inhalational anaesthetic desflurane, hypnotic propofol, analgesic fentanyl. A possibility to use the interface for measurement of desflurane and propofol absolute concentration in blood plasma and cerebrospinal fluid was demonstrated for the study of blood-brain barrier (BBB) properties.