Inhibitory effect of polysulfated heparin endostatin on alkali burn induced corneal neovascularization in rabbits

• 作者: Guo-Ying Mu Lei Liu Li-Jun Cao Ming Hu Ming-Xu Ge Ya-Li Zhang Zhao-Na Li Zhong-Fang Yuan
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: polysulfated HEPARIN ENDOSTATIN CORNEAL NEOVASCULARIZATION chemical BURNS RABBITS
• 年，卷（期）: 2015,（2）
• 页码范围: 234-238
• 页数: 5页
• 分类号: R779.13

摘要

AIM: To investigate anti-angiogenic effects of polysulfated heparin endostatin(PSH-ES) on alkali burn induced corneal neovascularization(NV) in rabbits.METHODS: An alkali burn was made on rabbit corneas to induce corneal NV in the right eye of 24 rabbits. One day after burn creation, a 0.2 m L subconjunctival injection of 50 μg/m L PSH-ES, 50 μg/m L recombinant endostatin(ES), or normal saline was administered every other day for a total of 14d(7 injections). Histology and immunohistochemisty were used to examine corneas.Corneal NV growth was evaluated as microvessel quantity and corneal vascular endothelial growth factor(VEGF)expression was measured by immunohistochemical assay.RESULTS: Subconjunctival injection of ES and PSHES resulted in significant corneal NV suppression, but PSH-ES had a more powerful anti-angiogenic effect than ES. Mean VEGF concentration in PSH-ES treated corneas was significantly lower than in ES treated and saline treated corneas. Histological examination showed that corneas treated with either PSH-ES or ES had significantly fewer microvessels than eyes treated with saline. Additionally corneas treated with PSH-ES had significantly fewer microvessels than corneas treated with ES.CONCLUSION: Both PSH-ES and recombinant ES effectively inhibit corneal NV induced by alkali burn.However, PSH-ES is a more powerful anti-angiogenic agent than ES. This research has the potential to provide a new treatment option for preventing and treating corneal NV.