Mechanisms of fertilization elucidated by gene-manipulated animals

• 来源期刊: 亚洲男性学杂志（英文版）
• 年，卷（期）: 2015,（4）
• 页码范围: 646-652
• 页数: 7页
• 语种: 中文
• DOI: 10.4103/1008-682X.153299

摘要

Capacitation and the acrosome reaction are key phenomena in mammalian fertilization.These phenomena were found more than 60 years ago.However,fundamental questions regarding the nature ofcapacitation and the timing of the acrosome reaction remain unsolved.Factors were postulated over time,but as their roles were not verified by gene-disruption experiments,widely accepted notions concerning the mechanism of fertilization are facing modifications.Today,although in vitro fertilization systems remain our central research tool,the importance of in vivo observations must be revisited.Here,primarily focusing on our own research,I summarize how in vivo observations using gene-manipulated animals have ducidated new concepts in the mechanisms of fertilization.