Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2015,（2）
• 页码范围: 262-265
• 页数: 4页
• 语种: 中文
• DOI: 10.1038/cr.2014.167

摘要

Dear Editor, Precise gene targeting in monkeys will substantially facilitate generation of human disease models [1,2].Although several transgenic monkey models have been successfully generated [3-6],most of past endeavors in gene targeting using primates had unfortunately failed.The usage of reprogrammable endonucleases,such as ZFN (zinc-finger nuclease),TALEN (transcription activator-like effector nuclease) and CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system,has opened new avenues for precise gene modification in monkeys [1,2].Among these,the CRISPR/Cas9 system has shown to be most efficacious in gene targeting based on its successful application in a number of animal species [7,8].