Danhong Huayu Koufuye combined with metformin attenuated diabetic retinopathy in Zucker diabetic fatty rats

• 作者: Bao-Qin Lin Jun-Li Lin Lu-Yun Hu Wen-Pei Chen Yan Ma Yan-Dong Wang Zi-Yang Zhang
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: 糖尿病的 retinopathy Danhong Huayu Koufuye METFORMIN Zucker 糖尿病的丰满的老鼠 antihyperglycemic 活动 氧化应力
• 年，卷（期）: 2015,（6）
• 页码范围: 1094-1100
• 页数: 0页
• 分类号: R587.2

摘要

AIM: To evaluate effects of Danhong Huayu Koufuye(DHK, a Chinese medicinal formulae) alone or combined with metformin on diabetic retinopathy(DR) in Zucker diabetic fatty(ZDF) rats, an animal model of obese type-2 diabetes, and then to investigate the mechanisms.·METHODS: ZDF(fa/fa) rats were administered with vehicle(distilled water), metformin, DHK, and DHK plus metformin. Electrophysiological and histological analysis were applied to evaluated effects of DHK alone or combined with metformin on DR. The levels of fasting blood glucose(FBG) and glycosylated hemoglobin(Hb A1c) in blood were measured to evaluate the antihyperglycemic activity of DHK. Furthermore, levels of nitric oxide(NO), malondialdehyde(MDA) and activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) in serum were measured to study effects of DHK on oxidative stress in ZDF rats. In addition, body weight, lipidic indexes and insulin level were also assessed.·RESULTS: DHK combined with metformin significantly reversed the prolongation of latency times of flash electroretinogram(FERG) and oscillatory potentials(OPs)in diabetic rats. Furthermore, DHK alone or combined with metformin showed a remarkable suppression of retinal neovascularization and amelioration of retinal internal limiting membrane morphology. Moreover, DHK alone or plus metformin reduced FBG(P <0.05), Hb A1c(P <0.01) and MDA(P <0.01) levels in diabetic rats. In addition, reductions in levels of triglycerides(TG)(P <0.01)and low density lipoprotein cholesterol(LDL-c)(P <0.01 and P <0.05, respectively) were also observed in diabetic rats treated with DHK alone or plus metformin.· CONCLUSION: DHK in combination with metformin had a preventive and therapeutic effect on DR in type-2diabetic rats, and the possible mechanisms may be alleviating hyperglycemia, reducing oxidative stress and improving lipid metabolism.