P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes

• 作者: Belinda L Curtis Lisa M Jurak Megan Faulks Reece A Sophocleous Ronald Sluyter Stephen J Curtis Tracey A Kuit
• 来源期刊: 世界血液学杂志
• 学科: 医学
• 关键词: Adenosine TRIPHOSPHATE Dog P2X1 RECEPTOR P2X7 RECEPTOR PHOSPHOLIPID PURINERGIC RECEPTOR Red blood cells
• 年，卷（期）: 2016,（4）
• 页码范围: 88-93
• 页数: 6页
• 分类号: R

摘要

AIM To determine if activation of the ATP-gated P2X7 receptor channel induces phosphatidylserine(PS)exposure in erythrocytes from multiple dog breeds.METHODS Peripheral blood was collected from 25 dogs representing 13 pedigrees and seven crossbreeds.ATP-induced PS exposure on canine erythrocytes in vitro was assessed using a flow cytometric Annexin V binding assay.RESULTS ATP induced PS exposure in erythrocytes from all dogs studied.ATP caused PS exposure in a concentrationdependent manner with an EC50 value of 395μmol/L.The non-P2X7 agonists,ADP or AMP,did not cause PS exposure.The P2X7 antagonist,AZ10606120,but not the P2X1 antagonist,NF449,blocked ATP-induced PS exposure.CONCLUSION The results indicate that ATP induces PS exposure in erythrocytes from various dog breeds and that this process is mediated by P2X7 activation.