Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2016,（1）
• 页码范围: 131-134
• 页数: 4页
• 语种: 中文
• DOI: 10.1038/cr.2015.132

摘要

Dear Editor, Mammalian haploid embryonic stem cells (haESCs) have been recently generated from parthenogenetic and androgenetic embryos [1, 2].Both parthenogenetic haESCs (PG-haESCs) and androgenetic haESCs (AG-haESCs) can be used for cell-based reverse and forward genetic screens on a whole-genome scale [3, 4].AG-haESCs, after intracytoplasmic injection into oocytes (referred to as ICAHCI), can be used as a sperm replacement to produce healthy semi-cloned (SC) mice at a rateof～2％ of transferred embryos [5, 6].