Metastasis-related genes in hepatocellular carcinoma cell-lines are clustered on chromosome territories predicted by transcriptome and proteome

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2016,（3）
• 页码范围: 380-382
• 页数: 3页
• 语种: 中文
• DOI: 10.1007/s11426-015-5478-7

摘要

Recently,we have analyzed expressional transcriptome and proteome for a number of cancer cell lines,including 8 hepatocellular carcinoma (HCC) ones [1,2].These 8 HCC cell lines consist of 6 metastatic (all with mutant p53) and 2 non-metastatic (with a mutant and a lost p53) ones,being all HBV traceable for relatively close genetic-backgrounds.We have analyzed the related dataset and obtained transcriptome (16353 genes) and proteome (7861 proteins) of these 8 HCC cell lines,and explored a group of up-regulated and down-regulated genes [3].Hereby we screened the differentially-expressed regions of transcript and protein at all chromosomes responsible for HCC metastasis,as shown in Figure 1,by Gene Set Enrichment Analysis (GSEA) [4].GSEA normalized enrichment score (NES) of each chromosome cytoband in metastatic versus non-metastatic HCC cell lines was provided in Table S1 (Supporting Information online).