Tracking translation of single mRNA molecule in live cells

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2016,（14）
• 页码范围: 1071-1073
• 页数: 3页
• 语种: 中文
• DOI: 10.1007/s11434-016-1116-9

摘要

Proteins are the major players in cellular functions.To produce proteins,genetic information stored in DNA needs to be firstly transcribed into mRNA,which is then translated by the ribosome.No doubt,protein expression can be regulated at both transcriptional and translational levels.Previous study has suggested that protein abundance ispredominantly controlled at translational level [1].Transcription and movements of individual mRNAs can be visualized and tracked in real time in vivo using fluorescent protein fused with mRNA stem-loop-binding proteins [2].However,methods to track translation at single molecule level in vivo have been lacking.On May 5th 2016,four independent groups [3-6] reported a set of novel tools to capture translational dynamics of individual mRNAs in live cells.