Non-interferometric photoacoustic remote sensing microscopy

• 来源期刊: 光：科学与应用（英文版）
• 关键词: all optical medical imaging optical resolution photoacoustic remote sensing
• 年，卷（期）: 2017,（2）
• 页码范围: 227-234
• 页数: 8页
• 语种: 英文
• DOI: 10.1038/lsa.2016.278

摘要

Elasto-optical refractive index modulation due to photoacoustic initial pressure transients produced significant reflection of a probe beam when the absorbing interface had an appreciable refractive index difference.This effect was harnessed in a new form of non-contact optical resolution photoacoustic microscopy called photoacoustic remote sensing microscopy.A noninterferometric system architecture with a low-coherence probe beam precludes detection of surface oscillations and other phase-modulation phenomenon.The probe beam was confocal with a scanned excitation beam to ensure detection of initial pressure-induced intensity reflections at the subsurface origin where pressures are largest.Phantom studies confirmed signal dependence on optical absorption,index contrast and excitation fluence.In vivo imaging of superficial microvasculature and melanoma tumors was demonstrated with ～ 2.7± 0.5 μm lateral resolution.