Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes

• 来源期刊: 生物物理学报
• 年，卷（期）: 2017,（4）
• 页码范围: 85-91
• 页数: 7页
• 语种: 中文

摘要

Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure targeting for obesity therapy.Here,we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester,which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms.Using this approach,we are able to demonstrate that the efficacy of norepinephrine-induced thermogenesis is low,and measure the maximum transient rate of temperature increase in brown adipocytes.