Heritable expansion of the genetic code in mouse and zebrafish

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2017,（2）
• 页码范围: 294-297
• 页数: 4页
• 语种: 中文
• DOI: 10.1038/cr.2016.145

摘要

Dear Editor, The genetic code can be expanded to encode unnatural amino acids (Uaas) by introducing an orthogonal tRNA/aminoacyl-tRNA synthetase (aaRS) pair to decode a stop codon.Initially developed in Escherichia coli [1],this strategy has been proven generally applicable in eukaryotic cells [2] and in generating transgenic invertebrates capable of Uaa incorporation,including Caenorhabditis elegans [3] and Drosophila melanogaster [4].Although cells and tissues of mouse can be transduced transiently to incorporate Uaas [5],it remains unknown whether transgenic vertebrates with a heritable expanded genetic code system-wide can be generated,as it is unclear whether the biological complexity of vertebrates allows the introduction,maintenance and transmission of the newly introduced genetic material for code expansion.Here we for the first time report the generation of transgenic mice and zebrafish with an expanded genetic code,providing valuable vertebrate model animals for biological and biomedical research.