Huaiqihuang Granules(槐杞黄颗粒) Reduce Proteinuria by Enhancing Nephrin Expression and Regulating Necrosis Factor κB Signaling Pathway in Adriamycin-Induced Nephropathy

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2017,（4）
• 页码范围: 279-287
• 页数: 9页
• 语种: 英文
• DOI: 10.1007/s11655-015-2293-0

摘要

Objective:To investigate the effects of Huaiqihuang Granules(槐杞黄颗粒,HQH),a mixture of Chinese herbs including Trametes robiniophila Murr,Fructus Lycii and Polygonatum sibiricum,on adriamycininduced nephropathy (ADRN) in rats and its underlying mechanisms.Methods:Rats with ADRN were divided into four groups:the sham group,the model group (distilled water),the low-dose HQH-treated (2 g/kg) group,and the high-dose HQH-treated (4 g/kg) group.Body weight and 24-h urinary protein (Upro) were checked every week.After 5-week intervention,at the end of the study,the rats were sacrificed and blood samples were collected for examination of biochemical parameters,including glomerular morphological makers,podocyte shape,cellular apoptosis,expressions of nephrin,inflammatory and apoptosis markers.Results:HQH ameliorated the rat's general status,proteinuria,renal morphological appearance and glomerulosclerosis.The decreased expression of nephrin in ADRN rats was increased by HQH,as well as the impaired podocyte foot process fusion.Cytosolic levels of p65 and inhibitor of nuclear factor κ B α (I κ B α) were decreased in ADRN rats,and recovered by the treatment of HQH.Consistently,the induced expression of tumor necrosis factor α (TNF-α),phosphorylated nuclear factor κ B p65 (p-NF κ B p65) and I κ B α in ADRN were markedly suppressed by HQH.In addition,induction of Bax,cleaved caspase-3 and cytochrome C in ADRN rats were suppressed by HQH,indicating the amelioration of apoptosis.Conclusion:HQH could ameliorate renal impairments in ADRN rats by increasing nephrin expression,inhibiting NF-κ B signaling pathway via the down-regulation of p-NF-κ B p65 and p-I κ Bα,and suppression of glomerular and tubular apoptosis.