Structural analysis of recombinant human ubiquitin-conjugating enzyme UbcH5c

• 来源期刊: 药学学报（英文版）
• 关键词: UbcH5c NF-κB Ubiquitination Ubiquitin-conjugating enzyme Crystal structure Inflammatory target
• 年，卷（期）: 2017,（3）
• 页码范围: 390-394
• 页数: 5页
• 语种: 英文

摘要

UbcH5c belongs to the ubiquitin-conjugating enzyme family and plays an important role in catalyzing ubiquitination during TNF-α-triggered NF-κB activation.Therefore,UbcH5c is a potent therapeutic target for the treatment of inflammatory and autoimmune diseases induced by aberrant activation of NF-κB.In this study,we established a stable expression system for recombinant UbcH5c and solved the crystal structure of UbcH5c belonging to space group P22121 with one molecule in the asymmetric unit.This study provides the basis for further study of UbcH5c including the design of UbcH5c inhibitors.