Isochlorogenic acid A promotes melanin synthesis in B16 cell through the β-catenin signal pathway

• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: isochlorogenic acid A melanogenesis β-catenin β-catenin signal pathway
• 年，卷（期）: 2017,（9）
• 页码范围: 800-807
• 页数: 8页
• 语种: 英文
• DOI: 10.1093/abbs/gmx072

摘要

Isochlorogenic acid A,also called 3,5-dicaffeoylquinic acid (3,5-diCQA),is a widespread phenolic compound in the plant.Recent studies have shown that it has antioxidant and anti-inflammatory activity.In addition,oxidative stress and inflammation induced by solar ultraviolet radiation is a very significant reason for skin depigmentation.Therefore,in this study,we evaluated the effect of 3,5-diCQA on B16 cells and explored its molecular mechanism.Results showed that 3,5-diCQA upregulated intracellular melanin production in a time-and dose-dependent manner.Tyrosinase (TYR) activity was also increased after treatment with 3,5-diCQA in a dose-dependent manner.Expressions of TYR,TYR-related protein1,TYR-related protein2,and microphthalmia-associated transcription factor were upregulated in a dose-dependent manner after 48 h of treatment with 3,5-diCQA.Results also showed that 3,5-diCQA promoted the phosphorylation of Akt at Thr308 and glycogen synthase kinase-3β at Ser 9.Moreover,3,5-diCQA increased the content of β-catenin in cell cytoplasm and nucleus by reducing the content of phosphorylated β-catenin (p-β-catenin).All these results suggest that 3,5-diCQA may mediate the acceleration of melanin synthesis by the β-catenin signal pathway.