Pigment epithelium derived factor (PEDF) prevents methyl methacrylate monomer-induced cytotoxicity in H9c2 cells

• 来源期刊: 生物医学研究杂志（英文版）
• 学科: 医学
• 年，卷（期）: 2017,（6）
• 页码范围: 512-520
• 页数: 9页
• 分类号: R681.5+7
• 语种: 英文

摘要

Acrylic bone cements are currently the most frequently and extensively used materials in orthopedic implant treatment.However,adverse effects have been described of acrylic bone cement on the cardiovascular system.In the present study,we examined the cytotoxicity of bone cement ingredient methyl methacrylate (MMA) to cardiomyocytes and the potential detoxifying effect of pigment epithelium-derived factor (PEDF) in H9c2 cells.We found that high concentration ofMMA (＞ 120 mmol/L) led to necrotic cell death in H9c2 cells.However,MMA at low concentrations (30-90 mmol/L) caused apoptosis.Pretreatment of PEDF prevented MMA-induced cytotoxicity.In addition,PEDF enhanced total superoxide dismutase activities,and decreased MMA-induced production ofmalonaldehyde.Furthermore,MMA-induced downregulation of Akt activity was suppressed by PEDF.PEDF also increased the levels ofperoxisome proliferator activated receptor gamma (PPARγ) and lysophosphatidic acids (LPA) through PEDF receptor.These results indicated that PEDF inhibited MMA-induced cytotoxicity through attenuating oxidative stress,activating the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and/or PEDF receptorLPA-PPARγ pathways in H9c2 cells.PEDF may be explored as a candidate therapeutic agent for alleviating bone cement implantation syndrome during orthopedic surgery.