Polymyxin B as an inhibitor of lipopolysaccharides contamination of herb crude polysaccharides in mononuclear cells

• 来源期刊: 中国天然药物
• 学科: 医学
• 年，卷（期）: 2017,（7）
• 页码范围: 487-494
• 页数: 8页
• 分类号: R965
• 语种: 英文
• DOI: 10.3724/SP.J.1009.2017.00487

摘要

Lipopolysaccharides (LPS) contamination in herbal crude polysaccharides is inevitable.The present study was performed to explore the effect of polymyxin B on abolishing the influence of LPS contamination in mononuclear cells.LPS was pretreated with polymyxin B sulfate (PB) at different concentrations for 1,5 or 24 h,and then used to stimulate RAW264.7 and mouse peritoneal macrophages (MPMs).The nitric oxide (NO) and tumor necrosis factor-α (TNF-αt) in cell culture supematant,as the indications of cell response,were assayed.Bupleurum chinensis polysaccharides (BCPs) with trace amount contamination of LPS was treated with PB.30 μg·mL-1 of PB,treating LPS (10 and 1 000 ng·mL-1 in stimulating RAW264.7 and MPMs respectively) at 37 ℃ for 24 h,successfully abolished the stimulating effect of LPS on the cells.When the cells were stimulated with LPS,BCPs further promoted NO production.However,pretreated with PB,BCPs showed a suppression of NO production in MPMs and no change in RAW264.7.In the in vitro experiments,LPS contamination in polysaccharide might bring a great interference in assessing the activity of drug.Pretreatment with PB (30 μg·mL-1) at 37 ℃ for 24 h was sufficient to abolish the effects of LPS contamination (10 and 1 000 ng·mL-1).