In vivo and in vitro anti-sepsis effects of physcion 8-O-β-glucopyranoside extracted from Rumex japonicus

• 来源期刊: 中国天然药物
• 学科: 医学
• 年，卷（期）: 2017,（7）
• 页码范围: 534-539
• 页数: 6页
• 分类号: R965
• 语种: 英文
• DOI: 10.3724/SP.J.1009.2017.00534

摘要

The present study was designed to investigate the anti-sepsis effects of physcion 8-O-β-glucopyranoside (POG) isolated from Rumexjaponicas and explore its possible pharmacological mechanisms.POG was extracted from R.japonicas by bioactivity-guided isolation with the anti-sepsis agents.Survival analysis in septic mouse induced by LPS and heat-killed Escherichia coli were used to evaluate the protective effect of POG (40 mg·kg-1,i.p.) on sepsis.Cytokines including TNF-α,IL-1β and IL-6 in RAW 264.7 cells induced by LPS (100 ng·mL-1) were determined by ELISA.In addition,the proteins expressions of TLR2 and TLR4 were determined by Western blotting assay.Our results demonstrated that POG (40 mg·kg-1,i.p.) possessed significant protective activity on the endotoxemic mice.The POG treatment (20,40,and 80 μg·mL-1) significantly decreased the TNF-α,IL-lβ and IL-6 induced by LPS (P ＜ 0.01) in a concentration-dependent manner.Furthermore,the TLR4 and TLR2 proteins were also down-regulated by POG at 20 (P ＜ 0.01),40 (P ＜ 0.01),and 80 μg.mL-1 (P ＜ 0.01).The present study demonstrated that the POG extracted from R.japonicas possessed significant anti-sepsis effect on endotoxemic mice,and can be developed as a novel drug for treating sepsis in the future.