SQLE induces epithelial-to-mesenchymal transition by regulating of miR-133b in esophageal squamous cell carcinoma

• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: esophageal squamous cell carcinoma miR-133b SQLE epithelial-to-mesenchymal transition
• 年，卷（期）: 2017,（2）
• 页码范围: 138-148
• 页数: 11页
• 语种: 英文
• DOI: 10.1093/abbs/gmw127

摘要

Increasing evidence suggests that microRNAs,which control gene expression at the posttranscriptional level,are aberrantly expressed in cancers and play significant roles in carcinogenesis and cancer progression.In this study,we show differential miR-133b down-expression in human esophageal squamous cell carcinoma (ESCC) cells and tissues.In addition,squalene epoxidase (SQLE),a key enzyme of cholesterol synthesis,is identified as the direct downstream target gene of miR-133b by luciferase gene reporter assay.Furthermore,ectogenic miR-133b expression and SQLE knockdown can inhibit proliferation,invasion,and metastasis,and diminish epithelialto-mesenchymal transition (EMT) traits of ESCC in vitro,implying that miR-133b-dependent SQLE can induce tumorigenicity and that SQLE is an EMT inducer.Xenograft experiment results also proved the biological function of SQLE in vivo.Therefore,we conclude that miR-133b-dependent SQLE plays a critical role in the potential metastasis mechanisms in ESCC.