Vascular protective effects of aqueous extracts of Tribulus terrestris on hypertensive endothelial injury

• 来源期刊: 中国天然药物
• 学科: 医学
• 年，卷（期）: 2017,（8）
• 页码范围: 606-614
• 页数: 9页
• 分类号: R965
• 语种: 英文
• DOI: 10.3724/SP.J.1009.2017.00606

摘要

Angiotensin Ⅱ (Ang Ⅱ) is involved in endothelium injury during the development of hypertension.Tribulus terrestris (TT) is used to treat hypertension,arteriosclerosis,and post-stroke syndrome in China.The present study aimed to determine the effects of aqueous TT extracts on endothelial injury in spontaneously hypertensive rats (SHRs) and its protective effects against Ang Ⅱ-induced injury in human umbilical vein endothelial cells (HUVECs).SHRs were administered intragastrically with TT (17.2 or 8.6 g·kg-1·d-1) for 6 weeks,using valsartan (13.5 mg·kg-1·d-1) as positive control.Blood pressure,heart rate,endothelial morphology of the thoracic aorta,serum levels of Ang Ⅱ,endothelin-1 (ET-1),superoxide dismutase (SOD) and malonaldehyde (MDA) were measured.The endothelial injury of HUVECs was induced by 2 × 10-6 mol·L-1 Ang Ⅱ.Cell Apoptosisapoptosis,intracellular reactive oxygen species (ROS) was assessed.Endothelial nitric oxide synthase (eNOS),ET-1,SOD,and MDA in the cell culture supernatant and cell migration were assayed.The expression of hypertension-linked genes and proteins were analyzed.TT decreased systolic pressure,diastolic pressure,mean arterial pressure and heart rate,improved endothelial integrity of thoracic aorta,and decreased serum leptin,Ang Ⅱ,ET-1,NPY,and Hcy,while increased NO in SHRs.TT suppressed Ang Ⅱ-induced HUVEC proliferation and apoptosis and prolonged the survival,and increased cell migration.TT regulated the ROS,and decreased mRNA expression ofAkt1,JAK2,PI3Ka,Erk2,FAK,and NF-κB p65 and protein expression of Erk2,FAK,and NF-κB p65.In conclusion,TT demonstrated anti-hypertensive and endothelial protective effects by regulating Erk2,FAK and NF-κBp65.