Anti-fibrotic Effects of Salvia miltiorrhiza and Ligustrazine Injection on LX-2 Cells Involved with Increased N-myc Downstream-Regulated Gene 2 Expression

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2017,（12）
• 页码范围: 923-928
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11655-016-2640-9

摘要

Objective:To investigate the effects of Salvia miltiorrhiza and Ligustrazine Injection (SML) on proliferation and apoptosis of human hepatic stellate cell LX-2 and the expression of N-myc downstreamregulated gene 2 (NDRG2,a tumor suppressor gene).Methods:HSCs from the LX-2 cell line were cultured in vitro.The proliferative state of different initial LX-2 cell numbers was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay.LX-2 cells were plated in 96-well plates at an approximate density of 2.50 x 104 cells/mL and cultured for 24 h followed by the application of different concentrations of SML (1,2,4 and 8 μ L/mL).Cell proliferation was measured using the MT-T assay at 24 and 48 h.Apoptosis was detected by flow cytometry at 24 h.LX-2 cells were treated with different concentrations of SML and extracted with protein lysis buffer.The levels of NDRG2 and β-catenin were measured by Western blot.Results:With the exception of the 1 and 2 μ L/mL concentrations,4 and 8 p L/mL SML inhibited cell proliferation in a concentration-dependent manner at 24 and 48 h (P＜0.05).With the exception of the 1 and 2 μ L/mL concentrations,the NDRG2 expression level was greatly increased in a concentration-dependent manner.However,the level of β-catenin was unaffected.Conclusion:SML inhibit LX-2 cell proliferation in a concentration-dependent manner,and the mechanism may be associated with NDRG2 over-expression.