Cell cycle-dependent control of homologous recombination

• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: homologous recombination cell cycle DSB repair
• 年，卷（期）: 2017,（8）
• 页码范围: 655-668
• 页数: 14页
• 语种: 英文
• DOI: 10.1093/abbs/gmx055

摘要

DNA double-strand breaks (DSBs) are among the most deleterious type of DNA lesions threatening genome integrity.Homologous recombination (HR) and non-homologous end joining (NHEJ) are two major pathways to repair DSBs.HR requires a homologous template to direct DNA repair,and is generally recognized as a high-fidelity pathway.In contrast,NHEJ directly seals broken ends,but the repair product is often accompanied by sequence alterations.The choice of repair pathways is strictly controlled by the cell cycle.The occurrence of HR is restricted to late S to G2 phases while NHEJ operates predominantly in G1 phase,although it can act throughout most of the cell cycle.Deregulation of repair pathway choice can result in genotoxic consequences associated with cancers.How the cell cycle regulates the choice of HR and NHEJ has been extensively studied in the past decade.In this review,we will focus on the current progresses on how HR is controlled by the cell cycle in both Saccharomyces cerevisiae and mammals.Particular attention will be given to how cyclin-dependent kinases modulate DSB end resection,DNA damage checkpoint signaling,repair and processing of recombination intermediates.In addition,we will discuss recent findings on how HR is repressed in G1 and M phases by the cell cycle.