Application of CRISPR/Cas9 in plant biology

• 来源期刊: 药学学报（英文版）
• 关键词: CRISPR/Cas system Gene editing technology Gene modification Plant biology Transcriptional regulation
• 年，卷（期）: 2017,（3）
• 页码范围: 292-302
• 页数: 11页
• 语种: 英文

摘要

The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates.It was developed as a gene editing technology in 2013.Over the subsequent years,it has received extensive attention owing to its easy manipulation,high efficiency,and wide application in gene mutation and transcriptional regulation in mammals and plants.The process of CRISPR/Cas is optimized constantly and its application has also expanded dramatically.Therefore,CRISPR/Cas is considered a revolutionary technology in plant biology.Here,we introduce the mechanism of the type Ⅱ CRISPR/Cas called CRISPR/Cas9,update its recent advances in various applications in plants,and discuss its future prospects to provide an argument for its use in the study of medicinal plants.