Single-molecule analysis in an electrochemical confined space

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2017,（9）
• 页码范围: 1187-1190
• 页数: 4页
• 语种: 英文
• DOI: 10.1007/s11426-017-9082-5

摘要

Electrochemical analysis of single molecules is a method with the strong ability of the enhanced efficiency and ultra-sensitivity.Here,we demonstrate that the electrochemical confined space could efficiently convert single molecule characteristics into measurable electrochemical signatures with high temporal resolution.The human telomere repeat sequence T8 was used as a probe to determine the electrochemical confined effect in a nanopore.Our results show that the nanopore with comparable confined space of the telomere repeat sequence exhibits the most distinguishable single-molecule signals which suggest the folded conformation of T8.This method will greatly extend the lifetime of a metastable conformation for a single biomolecule by strong analyte-nanopore interactions,which brings the new insight into the understanding of the biomolecule's function at single-molecule level.