Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2017,（10）
• 页码范围: 686-692
• 页数: 7页
• 语种: 英文

摘要

Layered double hydroxides (LDHs) are effective molecular carriers in cytological research,gene therapy,and transgenic applications.Herein,we investigated the internalization behavior of the LDH-DNA bioconjugates via a microscopic approach and analyzed the internalization pathway by dissipative particle dynamics (DPD) simulations.We experimentally found that LDH can efficiently carry DNA into the nucleus of cell in BY-2 suspension cells.Furthermore,atomic force microscopy and X-ray diffraction analysis demonstrated that the LDH-DNA bioconjugates mainly exist as a DNA-LDH-DNA sandwich complex,while the LDH-DNA-LDH sandwich complex and DNA-LDH complex cannot be excluded.The DPD simulations further indicated that only the DNA-LDH-DNA sandwich structure could penetrate the plasma membrane (PM),while PM is impermeable to the LDH-DNA-LDH sandwich complex and the DNA-LDH complex.This work provides novel perspective for understanding the membrane penetration mechanism of LDH nano-sheets and new insights into the design of novel molecular delivery systems.