TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in Streptomyces aureofaciens

• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: 6-demethylchlortetracycline Streptomyces aureofaciens biosynthetic gene cluster transformation-associated recombination
• 年，卷（期）: 2017,（12）
• 页码范围: 1129-1134
• 页数: 6页
• 语种: 英文
• DOI: 10.1093/abbs/gmx110

摘要

6-Demethylchlortetracycline (6-DCT),a tetracycline antibiotic produced by Streptomyces aureofaciens,is a crucial precursor employed for the semi-synthesis of tigecycline,minocycline,and amadacyclin (PTK 0796).In this study,the 6-DCT biosynthetic gene cluster (BGC) was cloned from genomic DNA of a high 6-DCT-producing strain,S.aureofaciens DM-1,using the transformation-associated recombination method.An extra copy of the 6-DCT BGC was introduced and integrated into the chromosome of S.aureofaciens DM-1.Duplication of the 6-DCT BGC resulted in a maximum increase of the 6-DCT titer by 34％.