Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2017,（2）
• 页码范围: 298-301
• 页数: 4页
• 语种: 中文
• DOI: 10.1038/cr.2017.9

摘要

Dear Editor, Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology.For example,the copy number of a particular chromosome in cancer cells is often abnormal (e.g.,more than two),and therefore probing chromosome copy numbers can aid cancer diagnosis.During interphase,each chromosome exists in its own territory in the nucleus,which can be imaged by fluorescence in situ hybridization (FISH) using sequence-specific probes of different colors [1,2].However,such chromosome painting has only been possible in fixed cells,and is not suitable for dynamic monitoring of live cells.Therefore,it would be valuable to visualize DNA replication of one chromosome during interphase,and follow chromosome dynamics in the M phase.