Spiral volumetric optoacoustic tomography visualizes multi-scale dynamics in mice

• 来源期刊: 光：科学与应用（英文版）
• 关键词: multi-scale dynamics multi-spectral imaging optoacoustic tomography real-time imaging whole-body imaging
• 年，卷（期）: 2017,（2）
• 页码范围: 103-110
• 页数: 8页
• 语种: 英文
• DOI: 10.1038/lsa.2016.247

摘要

Imaging dynamics at different temporal and spatial scales is essential for understanding the biological complexity of living organisms,disease state and progression.Optoacoustic imaging has been shown to offer exclusive applicability across multiple scales with excellent optical contrast and high resolution in deep-tissue observations.Yet,efficient visualization of multi-scale dynamics remained difficult with state-of-the-art systems due to inefficient trade-offs between image acquisition time and effective field of view.Herein,we introduce the spiral volumetric optoacoustic tomography technique that provides spectrally enriched highresolution contrast across multiple spatiotemporal scales.In vivo experiments in mice demonstrate a wide range of dynamic imaging capabilities,from three-dimensional high-frame-rate visualization of moving organs and contrast agent kinetics in selected areas to whole-body longitudinal studies with unprecedented image quality.The newly introduced paradigm shift in imaging of multi-scale dynamics adds to the multifarious advantages provided by the optoacoustic technology for structural,functional and molecular imaging.