Inhibitory Effect of the Extract of Phellodendron amurense Ruprecht Root on Collagen-Induced Arthritis in Mice

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2017,（10）
• 页码范围: 755-762
• 页数: 8页
• 语种: 英文
• DOI: 10.1007/s11655-016-2094-5

摘要

Objective:To investigate whether the dried root of Phellodendron amurenee Ruprecht (Phellodendri cortex;PC) extract improves arthritic symptoms through anti-inflammatory and immune-modulatory effects in collagen-induced arthritis in mice.Methods:Rheumatoid arthritis (RA) was induced in male DBA/1 mice by immunization with type Ⅱ collagen (Col Ⅱ).CIA mice were divided into 5 groups (n=10 per a group) with normal,CIA control,PC extract (50 mg/kg and 100 mg/kg)-treated,and meloxicam (50 mg/kg)-traated as the reference drug.The PC extract or meloxicam were administered orally in CIA mice once a day for 14 days after arthritis induction.Arthritic score,levels of anti-Col Ⅱ IgG2a antibody,prostaglandin E2 (PGE2),tumor necrosis factor (TNF)-α,and interleukin (IL)-17 in the sera of CIA mice were measured.Histopathological changes in the ankle joints of CIA mice were also analyzed by staining with hematoxylin and eosin (H and E),safranin-O and immunohistochemistry using anti-TNF-α and anti-lL-17 antibodies.Results:The arthritic score was increased in CIA mice in a time-dependent manner,as were the serum levels of anti-Col Ⅱ IgG2a antibody,PGE2,TNF-α,and IL-17.However,the oral administration of PC extract at 50 and 100 mg/kg in CIA mice significantly decreased the arthritic scores,and the serum levels of anti-Col Ⅱ IgG2a,PGE2,TNF-α,and IL-17 compared with those in the CIA group (P＜0.05 or P＜0.01).Furthermore,histopathological improvement of the joint architecture in CIA mice was observed after administration of PC extract.PC extract also significantly inhibited the expression of TNF-α and IL-17 in the joints of CIA mice by suppressing the expression of their mRNA and proteins.Conclusion:PC extract may improve the pathological progression of RA through the inhibition of joint destruction by synovial inflammation and immune-stimulation,therefore,it would be a potential anti-arthritic agent in RA.