Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2017,（10）
• 页码范围: 1305-1309
• 页数: 5页
• 语种: 英文
• DOI: 10.1007/s11426-016-9028-5

摘要

Stimulated emission depletion (STED) microscope is one of the most prominent super-resolution bio-imaging instruments,which holds great promise for ultrahigh-resolution imaging of cells.To construct a STED microscope,it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses.In this study,we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator.By using this method,almost all the confocal microscopes can be upgraded to a STED system without losing its original functions.After the pulse synchronization,our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging.