Scutellaria barbata D.Don Inhibits Colorectal Cancer Growth via Suppression of Wnt/β-Catenin Signaling Pathway

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2017,（11）
• 页码范围: 858-863
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11655-017-2775-3

摘要

Objective:To investigate the effect of the ethanol extract of Scutellaria barbata D.Don (EESB) on colorectal cancer (CRC) growth and Wnt/β-catenin signaling pathway in vivo and in vitro.Methods:In vivo experiment,CRC xenograft mouse model was constructed with injection of HT-29 cells.Following xenograft implantation,twenty mice were randomly divided into EESB-treated group (n=10) and control group (n=10) by a random number table,and were given with intra-gastric administration of 2 g/kg EESB or saline,5 days a week for 16 days,respectively.At the end of experiment,tumors were removed and weighed by electronic scales.The proliferation biomarker Ki-67 of tumor was evaluated by immunohistochemistry (IHC) assay.In vitro study,HT-29 cells were treated with 0,0.5,1.5,2.5 mg/mL EESB for 24 h.At the end of the treatment,the viability and survival of HT-29 cells were determined by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and colony formation assay,respectively.The mRNA expression of c-Myc,Survivin and adenomatous polyposis coli (APC) was examined by reverse transcription-polymerase chain reaction (RT-PCR) both in tumor tissues of CRC xenograft mice and HT-29 cells.Protein expression of c-Myc,Survivin,APC,and β-catenin as well as β-catenin phosphorylation level were evaluated by IHC assay or Western blotting.Results:EESB significantly reduced tumor weight in CRC xenografts mice,compared with the control group (P＜0.05).IHC assay showed that EESB significantly inhibited protein expression of Ki-67 in tumor tissues (P＜0.05).MTT assay showed that EESB significantly reduced HT-29 cell viability in a dose-dependent manner (P＜0.05).Colony formation assay showed that EESB dose-dependently decreased the survival of HT-29 cells (P＜0.05).In addition,RT-PCR assay showed that EESB decreased the mRNA expression of c-Myc and Survivin and increased APC expression,both in tumor tissues of CRC xenograft mice and HT-29 cells (P＜0.05).IHC assay or Western blotting showed that EESB decreased protein expression of β-catenin,c-Myc and Survivin,as well as increased APC expression and β-catenin phosphorylation in tumor tissues or HT-29 cells (P＜0.05).Conclusions:EESB significantly reduced tumor growth in CRC xenografts mice,and inhibited the viability and survival of HT-29 cells.EESB could suppress the activation of the Wnt/β-catenin pathway,which might be one of the mechanisms whereby Scutellaria barbata D.Don exerts its anticancer activity.