Sequence-specific recognition of HIV-1 DNA based upon nicking-assisted strand displacement amplification and triplex DNA

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2017,（11）
• 页码范围: 1468-1473
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11426-017-9112-3

摘要

Nucleic acid amplification test is a reliable method for primary human immunodeficiency virus (HIV) infection diagnosis.Herein,a novel fluorescent method for sequence-specific recognition of DNA fragment of HIV-1 was established based upon nicking-assisted strand displacement amplification (SDA) and triplex DNA.In the presence of target dsDNA,nicking-assisted SDA process generated a lot of ssDNA,which hybridized with molecular beacon to produce signal.The fluorescence intensity was proportional to the concentration of target dsDNA within the range from 5 to 1000 pmol/L,with a detection limit of 1.4 pmol/L.Moreover,it successfully distinguished target dsDNA from the nucleic acid extractive of human blood.Thus this method has the merit of high sensitivity,and it is suitable for sequence-specific recognition of target dsDNA in complex matrices,which made it a potential application in diagnosis of acquired immunodeficiency syndrome (AIDS) in the future.