High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2017,（3）
• 页码范围: 162-164
• 页数: 3页
• 语种: 中文

摘要

Genome editing plays an important role in the research of gene functions,gene correction and cell replacement therapy.Traditionally,genome editing is mainly dependent on the technology of homologous recombination (HR) and embryonic stem (ES) cell lines in mammals [1].However,genome editing is severely limited by the low efficiency of HR and lack of ES cell lines in most organisms.Recently,the development of zinc finger nucleases (ZFNs),transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) systems have made it simple and precise to perform genome editing in many species.