Gastrodin protects retinal ganglion cells through inhibiting microglial-mediated neuroinflammation in an acute ocular hypertension model

• 作者: Han Zhang Jia-Wei Wang Xiao-Fei Zhao Yao-Ming Liu
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 年，卷（期）: gjykzzywb_2017,（10）
• 页码范围: 1483-1489
• 页数: 7页
• 分类号: R77

摘要

AIM:To investigate the neuroprotective effect of gastrodin on retinal ganglion cells(RGCs) in an acute ocular hypertension(AOH) rat model and to identify its possible mechanism.METHODS:AOH rat model was performed in a randomly selected eye by anterior chamber perfusion and either received an intraperitoneal injection with various concentrations of gastrodin or normal saline.After 2 wk,the rats were sacrificed.Fluoro Gold was used to label survival RGCs.Immunostaining with anti-Iba1 in the retinal flat mounts to calculate the microglia density in the ganglion cell layer(GCL).Changes in microglial cytokines,tumour necrosis factor-alpha(TNF-α) and inducible NO synthase(i NOS) were examined with Western blot and reverse transcriptionquantitative polymerase chain reaction.Expression levels of total and phosphorylated p38 mitogen activated protein kinase(MAPK) were determined by Western blot.RESULTS:Results showed that AOH induced significant loss of RGCs and severe microglia activation in the GCL.Besides,AOH increased the phosphorylation of p38 MAPK and promoted the release of microglial cytokines in the retinas.Intraperitoneal injection with dose-dependent gastrodin significantly reduced the loss of RGCs and inhibited retinal microglia activation,accompanied with the decreased expression levels of microglial cytokines and p38 MAPK phosphorylation.CONCLUSION:Gastrodin exerts a neuroprotective effect on RGCs in an acute glaucoma animal model viainhibiting microglia activation and microglial-mediated neuroinflammation.The finding demonstrates the potential application of gastrodin in the neuroprotective therapy of acute glaucoma and other retinal neurodegenerative diseases characterized by microglia activation and RGCs death.