Complete Achilles Tenotomy: A New Improved Experimental Surgical Technique in Rats

• 作者: Clara Raposo Guilherme de Almeida Hanna Dias Jo?o da Costa Jorge Carvalho Kelly Biancardini Gomes Barbato Libardo Rodriguez Liszt Palmeira de Oliveira Renata Paiva
• 来源期刊: 动物科学期刊(英文)
• 学科: 医学
• 关键词: ACHILLES TENDON RUPTURE Animal EXPERIMENTATION Wound HEALING Collagen Elastic Tissue
• 年，卷（期）: 2018,（1）
• 页码范围: 1-11
• 页数: 11页
• 分类号: R73

摘要

Rupture of the Achilles tendon is one of the most frequent tendon injuries in humans. However, there is no standard surgical technique thus far to reproduce complete rupture of this tendon in rats. Surgical procedures were performed in Wistar rats under light microscopy, with posterior longitudinal incision and preservation of the plantaris tendon. The animals were sacrificed on 2nd and 8th postoperative week and we used hematoxilin and eosin, picrosirius-red and Weigert’s resorcin-fuchsin to observe general tendon healing. The mean operative time was one minute and 24 seconds. Histological studies showed cellular proliferation and fibrillogenesis at two weeks, with decreased amounts of cellularity and elastic fibers at the 8th week, besides changes in structural organization of collagen fibers. The complete Achilles tenotomy in rats, was improved under light microscopy and sparing the plantaris tendon. This surgical technique was simple and quick, showed signs of normal healing process, could be easily reproducible, and triggered translational researches in this area.