Graphene oxide has many carboxylic groups within its molecular structure, which is perfect for esterification and imidation, the derivatives graphene oxide GAP, GSO when reaction grapheme oxide with 2-amino pyridine (GAP) and 2-hydroxy-5-sulfobenzoic acid (GSO). These derivatives have been characterized by FTIR, H1 NMR, XRD and FSEM. The patterns of XRD calculated particles size using two equations and compared between them.