Polycaprolactone–carboxymethyl cellulose composites for manufacturing porous scaffolds by material extrusion

• 来源期刊: 生物设计与制造（英文版）
• 年，卷（期）: 2018,（4）
• 页码范围: 245-253
• 页数: 9页
• 语种: 中文

摘要

Polycaprolactone–carboxymethyl cellulose composites have been obtained and used to print porous structures by material extrusion. The materials used contained 0, 2 and 5％w/w of the carboxymethyl cellulose additive. These structures have been analyzed in terms of their morphology (including the evaluation of their porosity), mechanical properties under compression load and cell affinity. Cell affinity has been evaluated by culturing sheep mesenchymal stem cells and analyzing their viability by the Alamar Blue? assay at days 1, 3, 6 and 8. The results show that composites samples have similar values of porosity and apparent density than pure polycaprolactone ones. However, samples containing 5％w/w of carboxymethyl cellulose have micropores on the filaments due to a hindered deposition process. This characteristic affects the mechanical properties of the structures, so these ones have a mean compression modulus significantly lower than pure polycaprolactone scaffolds. However, the samples containing 2％w/w of carboxymethyl cellulose show no significant difference with the pure polycaprolactone ones in terms of their mechanical properties. Moreover, the presence of 2％w/w of additive improves cell proliferation on the surface of the porous structures. As complementary information, the flow properties of the composite materials were studied and the power law equations at 210 °C obtained, as this temperature was the 3D printing temperature. These equations can be useful for simulation and designing purposes of other manufacturing processes.