Expression and Role of Voltage-Gated Sodium Channels in Human Dorsal Root Ganglion Neurons with Special Focus on Nav1.7, Species Differences, and Regulation by Paclitaxel

• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2018,（1）
• 页码范围: 4-12
• 页数: 9页
• 语种: 英文

摘要

Voltage-gated sodium channels (Navs) play an important role in human pain sensation.However,the expression and role of Nav subtypes in native human sensory neurons are unclear.To address this issue,we obtained human dorsal root ganglion (hDRG) tissues from healthy donors.PCR analysis of seven DRG-expressed Nav subtypes revealed that the hDRG has higher expression of Nav 1.7 (～ 50％ of total Nay expression) and lower expression of Navl.8 (～ 12％),whereas the mouse DRG has higher expression of Nav 1.8 (～ 45％) and lower expression of Navl.7 (～ 18％).To mimic Nay regulation in chronic pain,we treated hDRG neurons in primary cultures with paclitaxel (0.1-1 μmol/L) for 24 h.Paclitaxel increased the Nav1.7 but not Nav1.8 expression and also increased the transient Na+ currents and action potential firing frequency in small-diameter (＜50 μm) hDRG neurons.Thus,the hDRG provides a translational model in which to study "human pain in a dish" and test new pain therapeutics.