Effects of leptin on femoral fracture in rats

• 来源期刊: 生物医学研究杂志（英文版）
• 学科: 医学
• 年，卷（期）: 2018,（2）
• 页码范围: 130-135
• 页数: 6页
• 分类号: R683.42
• 语种: 英文

摘要

In this study,our objective was to evaluate effects of leptin on fracture healing in rats.Seventy two male SpragueDawley (SD) rats were randomized into 3 groups.Standardized femoral fractures were created in all the rats.Group A was treated with 1 mL normal saline (NS),group B with 0.3 μg/kg leptin in 1 mL NS,and group C with 0.5 μg/kg leptin in 1 mL NS for 2 weeks intraperitoneally.Each group was divided into three subgroups including 8 rats for evaluation at 2,4 and 8 weeks.Radiological evaluation showed that callus formation of group B and C was all significantly higher than group A at 8 weeks (P=0.04 and P=0.013,respectively).There was no statistically significant difference in fracture healing between group B and group C at 8 weeks (P=0.197).Histological evaluation revealed fracture healing of group B and C was better than group A at 4 weeks (P =0.01 and P =0.002,respectively) and 8 weeks (P =0.008 and P =0.003,respectively).Micro-computed tomography (Micro-CT) analysis demonstrated that greater amounts of bony callus and evidence of bone fusion were observed in group B and C at 4 weeks (P =0.02 and P =0.04,respectively) and 8 weeks (P =0.005 and P =0.001,respectively) compared to group A.Group C also had better fracture healing than group B at 8 weeks (P =0.01).In conclusion,leptin has a positive effect on rat femoral fracture healing.