Pitavastatin attenuates AGEs-induced mitophagy vie inhibition of ROS generation in the mitochondria of cardiomyocytes

• 来源期刊: 生物医学研究杂志（英文版）
• 学科: 医学
• 年，卷（期）: 2018,（4）
• 页码范围: 281-287
• 页数: 7页
• 分类号: R392.3
• 语种: 英文

摘要

This study aimed to investigate whether pitavastatin protected against injury induced by advanced glycation end products products (AGEs) in neonatal rat cardiomyocytes,and to examine the underlying mechanisms.Cardiomyocytes of neonatal rats were incubated for 48 hours with AGEs (100 μ g/mL),receptor for advanced glycation end products (RAGE),antibody (1 μg/mL) and pitavastatin (600 ng/mL).The levels of p62 and beclinl were determined by Western blotting.Mitochondrial membrane potential (△Ψm) and the generation of reactive oxygen species (ROS) were measured through the JC-1 and DCFH-DA.In the AGEs group,the expression ofbeclinl was remarkably increased compared to the control group,while the expression of p62 was significantly decreased.AGEs also markedly decreased △Ψm and significantly increased ROS compared with the control group.After treatment with RAGE antibody or pitavastatin,the level ofbeclinl was markedly decreased compared with the AGEs group,but the level of p62 was remarkably increased.In the AGEs + RAGE antibody group and AGEs + pitavastatin group,△Ψm was significantly increased and ROS was remarkably decreased compared with the AGEs group.In conclusion,AGEs-RAGE may induce autophagy of cardiomyocytes by generation of ROS and pitavastatin could protect against AGEs-induced injury against cardiomyocytes.