香蕉枯萎病菌TR4原生质体转化和基因敲除体系构建

• 作者: 唐威华 张磊 王云月 郑泗军 郭燕
• 来源期刊: 植物病理学报
• 学科: 农学
• 年，卷（期）: 2018,（1）
• 所属期刊栏目: 研究简报
• 页码范围: 137-140
• 页数: 4页
• 分类号: S432.44
• 语种: 中文
• DOI: 10.13926/j.cnki.apps.000039

摘要

Fusarium wilt of banana,caused by Fusarium oxysporum f.sp.cubense tropic race 4 (Foc TR4),is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana industry.In order to reveal the infection process and pathogenesis of Foc TR4,the young mycelia (66.7 mg · mL-1) of wild-type strain of Foc TR4 (WT-FOC TR4) cultured for 18-20 h were lysed with enzyme mixture for protoplasting,which consisted of 25 mg · mL-1driselase,0.4 mg · mL-1 chitinase,15 mg · mL-1 lysing enzyme and 1.2 mol · L-1 potassium chloride.The resulted protoplasts of 2×107cells · mL-1 were used to test the efficiency of transformation mediated by polyethylene glycol,and up to 9 transformants per μg DNA were obtained.AmCyan,RFP and YFP gene were stably transferred into WT-Foc TR4,separately,using the protoplasts transformation system.The gene FoOCH1 encoding α-1,6-mannosyltransferase in WT-Foc TR4 was knocked out using the split-marker recombination technology.The genetic transformation and gene knockout system in this pathogen laid the foundation for the study of functional genomics and plant-pathogen interactions.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	张磊	中国科学院上海生命科学研究院	307	3154	27.0	45.0
2	郭燕	中国科学院上海生命科学研究院	13	22	2.0	4.0
3	唐威华	中国科学院上海生命科学研究院	2	3	1.0	1.0
4	王云月	中国科学院上海生命科学研究院	1	0	0.0	0.0
5	郑泗军	中国科学院上海生命科学研究院	1	0	0.0	0.0