Protective Effects of Danlou Tablet(丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2018,（8）
• 页码范围: 613-620
• 页数: 8页
• 语种: 英文
• DOI: 10.1007/s11655-016-2448-7

摘要

Objective:To observe the in vivo effect of Danlou Tablet(丹萎片,DLT) on myocardial ischemia andreperfusion (I/R) injury,Methods:DLT effects were evaluated in mouse heart preparation using 30-min coronaryocclusion followed by 24-h reperfusion and compared among sham group (n=6),I/R group (n=8),IPC group(ischemia preconditioning,n=6) and DLT group (I/R with DLT pretreatment for 3 days,750 mg·kg-1·day-1,n=8).Theeffects of DLT were characterized in infarction size (IS) compared with risk region (RR) and left ventricle using theEvans blue/triphenyltetrazolium chloride double dye staining method in vivo.Furthermore,the dose-dependenteffect of DLT on I/R injury was evaluated by double staining method.Five different concentrations of DLT (0.625,1.25,2.5,5 and 10 g·kg-1·day-1) were chosen in this study,and dose-response curve of DLT was obtained onthese data.Results:The ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than theI/R group (P＜0.05 or P＜0.01),the ratio of IS to RR was also reduced in the DLT and IPC groups (P＜0.01),whilethere were no differences in RR among the four groups (P＞0.05).Experiments showed incidence of arrhythmiaswas reduced in the DLT group (P＜0.01).Furthermore,DLT produced a dose-dependent inhibitory effect with a halfmaximal inhibitory concentration of 1.225 g·kg-1·day-1.Conclusions:Our research concluded that DLT was effectivein reducing I/R injury in mice,and provided experimental supports for the clinical use of DLT.