Sharpening emitter localization in front of a tuned mirror

• 来源期刊: 光：科学与应用（英文版）
• 年，卷（期）: 2018,（6）
• 页码范围: 671-678
• 页数: 8页
• 语种: 英文
• DOI: 10.1038/s41377-018-0104-z

摘要

Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1.Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2-4.Here,we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two.The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength,as experimentally demonstrated for dual-color SMLM in cells.