Monitoring disulfide bonds making and breaking in biological nanopore at single molecule level

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2018,（11）
• 页码范围: 1385-1388
• 页数: 4页
• 语种: 英文

摘要

Monitoring subtle changes in ionic current flow through a nanopore could be applied to observe single molecule reaction.Here,we introduced cysteine to substitute for lysine at position 238 constructing a mutant aerolysin K238C.It could be regarded as a nanoreactor to efficiently visualize chemical bonds making and breaking.The compound 5,5'-dithiobis-(2-nitrobenzoic acid)(DTNB) was selected as a reactant coming into collisions on the interface of the pore to occur a reversible reaction.Our results showed that the mutant aerolysin could respond to three molecules of DTNB simultaneously and reflect corresponding levels with distinguishable current signals.Therefore,this method constitutes a simple,generic tool for monitoring single molecule reaction,which evokes a guidance for the mutant aerolysin towards the application of tracking other more reactions at single molecule level.