Super-resolution imaging and real-time tracking lysosome in living cells by a fluorescent probe

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2018,（4）
• 页码范围: 483-489
• 页数: 7页
• 语种: 英文

摘要

Lysosomes function as important organelles within cells and their movement associates with diverse biological events,hence the real-time tracking of lysosomal movement is of great significance.However,since most lysosome fluorescent probes suffer from relatively unsatisfactory photostability,tracking lysosomal movement in real-time remains challenging.Here,we report that a naphthalimide-based fluorescent compound,namely NIMS,is a quite promising probe for lysosome imaging.The visualizing mechanism lies in the selective accumulation of NIMS in lysosomes via a protonation reaction,followed by the fluorescence enhancement due to the interactions of NIMS with proteins.Owing to its high selectivity and good photostability,NIMS was successfully applied to capture super-resolution fluorescence images of lysosomes.More importantly,real-time tracking of lysosome movement in a single living cell by NIMS was realized with a confocal laser scanning microscope.Surprisingly,even in normal culture conditions,around 2/3 of the captured lysosomes were observed to move within 5 min,indicative of the highly dynamic features of lysosomes.Thus,this probe may facilitate the understanding of the lysosome dynamics in physiological or pathological conditions.