Engineering a customized nanodrug delivery system at the cellular level for targeted cancer therapy

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2018,（4）
• 页码范围: 497-504
• 页数: 8页
• 语种: 英文

摘要

Drug administration customized to individual cells could intrinsically address cancer heterogeneity and provide a safe and effective method for delivering personalized treatment.To accomplish this,we developed a smart nanodrug delivery system characterized by cancer cell-targeted drug delivery and intracellular biomarker-responsive drug activation.This system was composed of a long-nicked DNA duplex formed by tandem hybridization of two extended antisense oligonucleotides whose ends were separately blocked with a cancer cell-specific aptamer,AS1411,and a replaceable anti-biomarker probe (ABP).We demonstrated that this DNA nanodrug was directed to cancer cells with the guidance power of AS1411 and then activated by the presence of a given intracellular biomarker.By using such a belt-and-braces strategy,this DNA nanodrug system could safely and efficiently accelerate apoptosis of target cancer cells.Moreover,since the expression level of biomarkers tends to indicate the specific physiological state of individual cells,biomarker-responsive activation of the nanodrug is expected to enable customized drug administration at the cellular level.