Targeted activation of BmCyclinE in Bombyx mori using designer TAL effectors

• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2019,（6）
• 页码范围: 1055-1058
• 页数: 4页
• 语种: 英文
• DOI: 10.1111/1744-7917.12613

摘要

Dear Editor, Artificial expression systems are crucial to functional genomics studies,constructing synthetic genetic circuits,and bioengineering (Werner & Gossen,2014).They are usually achieved by cloning the coding sequence (CDS) of a target gene into a vector containing natural or engineered regulatory elements.However,this strategy is limited when target genes are hard to clone,for example when the CDS is too long,or expression level is too low.Engineered transcription activator-like effector (TALE) proteins,which enable the targeting of custom-defined sequences with high efficiency and specificity,were developed to overcome this limitation.